Vol. 16, no.2, 2024
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MEDICAL PHYSICS
Designing a microchip for exosome isolation with the ability to simultaneously impregnate it with imatinib: an in vitro analysis
Amir Monfaredan, Elahe Motevaseli, Gholamreza Tavoosidana
Tehran University of Medical Sciences, Department of Molecular Medicine, School of Advanced Technologies in Medicine, http://en.tums.ac.ir/
Tehran 1416753955, Iran
E-mail: monfaredanamir@gmail.com, e_motevaseli@tums.ac.ir, g-tavoosi@tums.ac.ir
Fakher Rahim
Cihan University-Sulaimaniya, https://sulicihan.edu.krd/
Sulaimaniya 46001, Iraq
E-mail: rahim.fakher@sulicihan.edu.krd
Kudaiberdi G. Kozhobekov
Osh State University, https://oshsu.kg/ru/
Osh 723500, Kyrgyzstan
E-mail: kudayberdi.kozhobekov@oshsu.kg
Mohammad Hossein Modarressi
Tehran University of Medical Sciences, Department of Medical Genetics, Faculty of Medicine, http://en.tums.ac.ir/
Tehran 1416753955, Iran
E-mail: modaresi@sina.tums.ac.ir
Alaviyehsadat Hosseininasab
GeneDia Life science company, https://tracxn.com/
Tehran 13000-15000, Iran
E-mail: shrzd.hsni@gmail.com
Ali-Akbar Aghajani-Afrouzi
Payame Noor University, Department of Business Administration, https://pnu.ac.ir/
Tehran 19569, Iran
E-mail: aghajaniali3@gmail.com, mshafiees@sina.tums.ac.ir
Mahdi Shafiee Sabet
Tehran University of Medical Sciences, School of Medicine, http://en.tums.ac.ir/
Tehran 14167539559, Iran
E-mail: mshafiees@sina.tums.ac.ir
Received January 17, 2024, peer-reviewed January 18, 2024, accepted January 19, 2024, published April 25, 2024
Abstract: Exosomes, a small bilayer membrane derived from eukaryotic cells, have been identified as a useful natural delivery platform due to their suitable size, biocompatibility, structural stability, high loading capacity, and editable surface capability. Due to the difficulty of maintaining the highly pure exosome, several attempts have conducted the techniques for exosome isolation. In recent years, microstructures have found many applications in chemistry, biology, and medicine due to their high accuracy and low cost of materials. Soft lithography is a low-cost, fast, accurate, and yet widely used method of construction of Micron channels. In the present study, a soft lithography process has been performed to construct channels for exosome separation with immunoaffinity function. Both biochemical and biophysical categories tests were performed to examine the quality of extracted exosomes from different sources (serum, cell supernatant, and urine) and compared with the commercially available kit. Results showed that the current technique was capable to isolate exosomes with a high yield rate, purity, and low time consumption. All forms of the imatinib loaded exosomes exhibited the antitumor activity against KYO-1 cell line.
Keywords: personalized medicine; exosome; laboratory on a chip; leukemia; targeted therapy
UDC 61:53(075.9)
RENSIT, 2024, 16(2):223-238e DOI: 10.17725/j.rensit.2024.16.223
Full-text electronic version of this article - web site http://en.rensit.ru/vypuski/article/552/16(2)223-238e.pdf