Vol. 14, no.4, 2022


Methods for studying parameters biogenic metal nanoparticles, formed in situ

Dmitry A. Skladnev, Vladimir V. Sorokin

Vinogradsky Institute of Microbiology of RAS, http://www.inmi.ru/
Moscow 117312, Russian Federation
E-mail: skladda@gmail.com, vlvlsorokin@gmail.com
Sergey P. Karlov
Moscow Polytechnic University, http://mospolytech.ru/
Moscow 107023, Russian Federation
E-mail: karlovsp@yandex.ru
Vladimir I. Anisimkin
Kotelnikov Institute of Radioengineering and Electronics RAS, http://www.cplire.ru/
Moscow 125009, Russian Federation
E-mail: anis@cplire.ru

Received 15 November 2022, peer-reviewed 22 November 2022, accepted 29 November 2022

Abstract:: We propose an innovative method for assessing the integral level of metabolic activity of biological objects, based on the natural ability of living cells for generation metal nanoparticles from cations sources (artificially introduced into the samples), while in sterile samples or in the presence of inactive cells, the formation of nanoparticles does not occur. The method is called DBNG (detection of biogenic nanoparticles generation/growth). This article presents several examples of the use of the main analytical approaches applicable to obtain information about the parameters of biogenic nanocrystalline structures that formed in situ(directly in the tested samples), as well as options for the practical use of the proposed nanobiotechnological innovative approach DBNG to assess (estimate) the basic integral properties of living cells.

Keywords: formation of de novo nanoparticles, detection of biogenic metal nanoparticles, metabolic activity of cells, fluorescence spectrometry, acoustoelectronic technologies

UDC 57.016.6 +57.033

RENSIT, 2022, 14(3):393-414e DOI: 10.17725/rensit.2022.14.393.

Full-text electronic version of this article - web site http://en.rensit.ru/vypuski/article/470/14(4)393-414e.pdf