Vol. 11, no.3, 2019
РусскийEnglish
NANOSYSTEMS
BINDING OF SHORT DNA OLIGONUCLEIDES TO THE SURFACE OF REDUCED GRAPHENE OXIDE TO CREATE BIOLOGICAL SENSORS
Ivan A. Komarov, Alexander N. Kalinnikov, Sergey N. Scherbin, Vyacheslav A. Seleznev
Bauman Moscow State Technical University, http://www.bmstu.ru/
Moscow 105005, Russian Federation
E-mail: ikomarov@emtc.ru, alexandr.kalinnikov@emtc.ru, sshcherbin@emtc.ru, vseleznev@emtc.ru
Olga M. Antipova
Lomonosov Moscow State University, https://www.msu.ru/
Moscow 119991, Russian Federation
E-mail: antipovachem@gmail.com
Nikolay S. Struchkov
National Research University of Electronic Technology, https://miet.ru/
Moscow 124498, Zelenograd, Russian Federation
E-mail: struchkov.nikolaj@gmail.com
Received 31.10.2019, peer reviewed 11.11.2019, accepted 18.11.2019
Abstract. The study examined the possibility of immobilization of short DNA oligonucleotides (aptmers) on the surface of reduced graphene oxide, with the aim of forming a bio-sensitive sensory layer. The features of laser reduction of graphene oxide were considered, and the parameters of reduction were determined, which were implicated in achieving partial reduction. The possibility of immobilization of aptamers on graphene oxide with a low degree of reduction and the response of sensory structures formed on the basis of this basis is shown. A significant difference between the response of the sensors to the effects of thrombin (target protein) and albumin (comparison protein) was demonstrated.
Keywords:aptamers, biosensors, immobilization, nucleotides, graphene oxide
UDC 620.3
RENSIT, 2019, 11(2):321-330 DOI: 10.17725/rensit.2019.11.321
Full-text electronic version of this article - web site http://en.rensit.ru/vypuski/article/297/11(3)321-330e.pdf