Vol. 6, №1, 2014

NANOSYSTEMS

MOLECULAR NANOBIOSENSOR ON THE BASIS OF GLUCOSE OXYDAS ENZYME
Kashin V.V., Kolesov V.V., Krupenin S.V.
Kotel’nikov Institute of Radio Engineering and Electronics of RAS, http://www. cplire.ru
kvv@cplire.ru
Parshintsev A.A., Soldatov E.S.
Lomonosov Moscow State University, Physics Department, http://www.phys.msu.ru
esold@phys.msu.ru
Reshetilov A.N.
Institute of Biochemistry and Physiology of microorganisms of RAS, http://www.ibpm.ru
reshetilov@ibpm.pishchino.ru
Azev V.N.
Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of RAS, http://www.bibc.psn.ru
azev@bibc.psn.ru

Received 16.12.2013
The last achievements in a solution of the miniaturization problem of electronic devices, and also successes in combination of technologies of biology and nanoelectronics allowed to develop original designs of nanodevices, in particular, transistors on the basis of single molecules that gives essentially new possibility of use of nanostructures for development not only miniature physical devices, but also for the solution of more wide range of tasks in live systems. In the work the planar topology for the nanoelectronics transducer on the basis of the glucose oxydase enzyme is developed. On the basis of the methods of electron-beam nanolithography, two shadow deposition and plasma-chemical etching the planar nanostructures for the nanoelectronics transducer were prepared. The method of the chemical surface modification of silicon dioxide by epoxysilane was developed and the immobilization of the glucose oxidase enzyme on the surface of planar nanostructure for the nanoelectronics transducer through the linker molecules was realized. The registration procedure of the biochemical signals was developed. The electronic properties of a nanoelectronic transducer were investigated in buffer solution in absence and at addition of a glucose oxydas. It is shown that on a control sample reaction to glucose oxydas is absent, and on the nanoelectronic structure modified by enzyme an electronic response of the biosensor to 10 мМ solution of glucose is present. Thus a change in the functional state of the immobilized ferment of the glucose oxidase enzyme with the presence (oxidation) of glucose in the test solution was demonstrated.

Keywords: biosenor, nanotransistor, nanostructures, enzymatic electrochemical sensors, enzymatic activity.

UDC 544.6:57 (579.087.9+543.95):543.86

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