MOLECULAR NANOBIOSENSOR ON THE BASIS OF GLUCOSE OXYDAS ENZYME
Kashin V.V., Kolesov V.V., Krupenin S.V.
Kotel’nikov Institute of Radio Engineering and Electronics of RAS, http://www. cplire.ru
Parshintsev A.A., Soldatov E.S.
Lomonosov Moscow State University, Physics Department, http://www.phys.msu.ru
Institute of Biochemistry and Physiology of microorganisms of RAS, http://www.ibpm.ru
Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of RAS, http://www.bibc.psn.ru
The last achievements in a solution of the miniaturization problem of electronic devices, and also successes in combination of technologies of biology and nanoelectronics allowed to develop original designs of nanodevices, in particular, transistors on the basis of single molecules that gives essentially new possibility of use of nanostructures for development not only miniature physical devices, but also for the solution of more wide range of tasks in live systems. In the work the planar topology for the nanoelectronics transducer on the basis of the glucose oxydase enzyme is developed. On the basis of the methods of electron-beam nanolithography, two shadow deposition and plasma-chemical etching the planar nanostructures for the nanoelectronics transducer were prepared. The method of the chemical surface modification of silicon dioxide by epoxysilane was developed and the immobilization of the glucose oxidase enzyme on the surface of planar nanostructure for the nanoelectronics transducer through the linker molecules was realized. The registration procedure of the biochemical signals was developed. The electronic properties of a nanoelectronic transducer were investigated in buffer solution in absence and at addition of a glucose oxydas. It is shown that on a control sample reaction to glucose oxydas is absent, and on the nanoelectronic structure modified by enzyme an electronic response of the biosensor to 10 мМ solution of glucose is present. Thus a change in the functional state of the immobilized ferment of the glucose oxidase enzyme with the presence (oxidation) of glucose in the test solution was demonstrated.
Keywords: biosenor, nanotransistor, nanostructures, enzymatic electrochemical sensors, enzymatic activity.
UDC 544.6:57 (579.087.9+543.95):543.86
Bibliography - 29 references
Full-text electronic version of this article - web site http://elibrary.ru
- Reshetilov AN, Reshetilova TA. Nanosensors and their applications. In: Metal nanoparticles in microbiology. Berlin, Springer Verlag, 2011, p. 269-283.
- Asefa T, Duncan CT, Sharma KK. Recent advances in nanostructured chemosensors and biosensors. Analyst, 2009, 134(10):1980-1990.
- Soldatov ES, Khanin VV, Trifonov AS, Gubin SP, Kolesov VV, Presnov DE, Iakovenko SA, Khomutov GB, Korotkov AN. Room temperature molecular single-electron transistor. Physics Uspekhi, 1998, 41(2):202-202.
- Gubin SP, Gulayev YuV, Khomutov GB, Kislov VV, Kolesov VV, Soldatov ES, Sulaimankulov KS, Trifonov AS. Molecular clusters as building blocks for nanoelectronics: the first demonstration of cluster SET transistor at room temperature. Nanotechnology, 2002, 13:185-194.
- Soldatov ES, Gubin SP, Maximov IA, Khomutov GB, Kolesov VV, Sergeyev-Cherenkov AN, Shorokhov VV, Sulaimankulov KS, Suyatin DB. Molecular cluster based nanoelectronics. Microelectronic Engineering, 2003, 69:536-548.
- Gubin SP, Gulyaev YuV, Khomutov GB, Kislov VV, Kolesov VV, Maximov IA, Samuelson L, Soldatov ES, Taranov IV. Electronics of molecular nanoclusters. Intern. J. of Nanoscience, 2004, 3(1-2):137-147.
- Kolesov VV, Sapkov IV, Soldatov ES. Molecular single electron transistor on the basis of the planar nanostructure. Proc. 20-th Int. Crimean Conf. “Microwave & Telecommunication Technology” (CriMiCo’2010), 13-17 Sept. 2010, Sevastopol, Crimea, Ukraine, pp. 857-858.
- Scognamiglio V, Pezzotti G, Pezzotti I, Cano J, Buonasera K, Giannini D, Giardi MT. Biosensors for effective environmental and agrifood protection and commercialization: from research to market. Microchimica Acta, 2010, 170:215-225.
- Reshetilov AN, Kitova AE, Arkhipova AV. Determination of ethanol in acetic acid-containing samples by a biosensor based on immobilized Gluconobacter cells. Nusantara Bioscience. MBI & UNS Solo, 2012, 3:97-100.
- Pantelopoulos A, Bourbakis NG. A survey on wearable sensor-based systems for health monitoring and prognosis. IEEE Transactions on Systems, Man and Cybernetics, Part C: Applications and Reviews, 2009, 40(1):1-12.
- Reddy RRK, Chadha A, Bhattacharya E. Porous silicon based potentiometric triglyceride biosensor. Biosensors and Bioelectronics, 2001, 16(4-5):313-317.
- Adami M, Sartore M, Baldini E, Rossi A, Nicolini C. New measuring principle for LAPS devices. Sensors and Actuators B: Chemical, 1992, 9(1):25-31.
- Pan T, Huang M-D, Lin W-Y, Wu M-H. A urea biosensor based on pH-sensitive Sm2TiO5 electrolyte–insulator–semiconductor. Analytica Chimica Acta, 2010, 669:68-74.
- Bergveld P. Thirty years of ISFETOLOGY. What happened in the past 30 years and what may happen in the next 30 years. Sensors and Actuators B: Chemical, 2003, 88:1-20.
- Pourmand M, Karhanek M, Persson HHJ, Webb CD, Lee TH, Zahradníková A, DavisRW. Direct electrical detection of DNA synthesis. Proc. of the National Academy of Sciences of the United States of America, 2006, 103(17):6466-6470.
- Wu M, Lin T-W, Huang M-D, Wang H-Y, Pan T-M. Label-free detection of serum uric acid using novel high-k Sm2TiO5 membrane-based electrolyte–insulator–semiconductor. Sensors and Actuators B, 2010, 146:342–348.
- De Benedetto GE, Palmisano F, Zambonin PG. One-step fabrication of a bienzyme glucose sensor based on glucose oxidase and peroxidase immobilized onto a poly(pyrrole) modified glassy carbon electrode. Biosensors and Bioelectronics, 1996, 11(10):1001-1008.
- Krupenin VA, Lotkhov SV, Vishenski SV. Photo and electron-beam lithography sharing common stencil. J.Vac. Sci. Technol., 1993, B11(6):2132.
- Steinmann P, Weaver JMR. Fabrication of sub-5 nm gaps between metallic electrodes using conventionsl lithographic techniques. Vac.Sci.&Technol. B, 2004, 22:3178.
- Tolliver D, Novicky R, Hess D et al. VLSI Electronics Microstructure Science: Plasma Processing for VLSI. Einspruch NG, Braun D (eds.). Miami, Acad.Press, 1981.
- Steinmann P, Weaver JMR. Fabrication of sub-5 nm gaps between metallic electrodes using conventionsl lithographic techniques. Vac.Sci.&Technol. B, 2004, 22:3178-3180.
- Kolesov VV, Sapkov V, Soldatov ES. Using a Focused Ion Beam for the Creation of a Molecular Single Electron Transistor. Moscow Univ. Physics Bulletin, 2009, 64(4):384-388.
- Park H, Lim AKL, Alivisatos AP. Fabrication of metallic electrodes with nanometer separation with electromigration. Appl. Phys. Letters, 1999, 35:2-5.
- Trouwborst ML, van der Molen SJ, van Wees BJ. The role Joule heating in the formation of nanogaps by electromigration. J.Appl.Phys., 2006, 99:1143-1147.
- O’Neill K, Osorio EA, van der Zant HSJ. Self Breaking in Planar Few-atom Au Constrictions for nm-Spaced Electrodes. Appl. Phys. Lett., 2007, 90:1331-1336.
- Heersche HB, Lientschnig G et al. In situ imaging of electromigration-induced nanogap formation by transmission electron microscopy. Appl. Phys. Lett., 2007, 91(7):072107-3.
- Gulyaev YuV, Kolesov VV, Kislov VV, Taranov IV, Gubin SP, Khomutov GB, Soldatov ES, Maximov IA, Samuelson L. Electronics of molecular nanoclusters. Intern. J. of Nanoscience, 2004, 3(1-2):137-147.
- Bidey SP, Brodelius P, Kabral IMA, Kaflan MP. Immobilised cells and enzymes a practical approach. Woodward J (ed.). Oxford-Washington, IRL Press, 1985, 215 p.